What is a COA and how is peptide purity verified?
Learn how to read a lot-specific certificate of analysis and distinguish HPLC purity from mass-spectrometry identity testing.
What is a certificate of analysis?
A certificate of analysis, or COA, documents the tests and results reported for a material or lot. Check that its identity and lot match the vial. Look for the methods, specifications, results, and report details rather than relying on a purity number without context.
What does HPLC measure?
High-performance liquid chromatography separates components under a stated analytical method. A reported chromatographic purity value often reflects relative peak areas under that method’s detection conditions. It is not, by itself, proof of the quantity in a vial, sterility, or absence of every possible impurity.
What does mass spectrometry add?
Mass spectrometry measures mass-to-charge signals and can support molecular identity by comparison with the expected material. Identity and chromatographic purity answer different questions; evaluate them together with the test method and any other required analyses.
How can researchers request ENOS documentation?
Third-party tested. COAs available on request. Contact ENOS with the product identity and lot number, when available, to request the corresponding documentation. Read the actual report for its scope; do not infer unreported tests or results.
Frequently asked questions
Does an HPLC purity result prove sterility?
No. Chromatographic purity and sterility are different attributes and require different evidence.
Are ENOS COAs available?
Third-party tested. COAs available on request.
References
For laboratory research use only. Not for human or veterinary consumption.